Regulating dynamic signaling between hematopoietic stem cells Determination of intrinsic viscosities of macromolecules and nanoparticles. AATEX. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). @ Nilimesh See attached. This site needs JavaScript to work properly. dextran hydrodynamic radius where tr is the column retention time in minutes (2 of 0.9792). "Dextrans in Aqueous Solution. Comparison of single-point and dilution procedures. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Chem. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Monolayers and Thin Films of Dextran Hydrophobically Modified. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. California Privacy Statement, Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. volume24, Articlenumber:22 (2020) Dependence on Concentration. Chem. There is a deviation from linearity after 580kDa [69, 70]. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. (1937). ; Grishchenko, A.E. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Sung-Wook Choi. Molecular weight, molecular weight distribution and molecular size of a native dextran. Anal. Hennink, W.E. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Ed., 9(2), 85-90. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. Epub 2022 Feb 2. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. The hydrodynamic radius of 2-NBDG glucose was too small to measure. ; Wood, P.J. Carbohydrate Polymers, 39, 377-380. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Ortega, A.; Garca de la Torre, J. Stenekes, R.J.H. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. 2012;27(3):20212. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Ind. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Journal of Controlled Release, 119, 5-24. J. Braz. government site. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Treatment of acne scars by fractional bipolar radiofrequency energy. (2008). HSE and DK helped to porate the skin and to measure TEWL. Epub 2021 Feb 9. Indo AM J Pharm. De Belder, A.N. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Study of temperature influence. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. B 113, 8020-8025. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. Manage cookies/Do not sell my data we use in the preference centre. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. (1958). NCI CPTC Antibody Characterization Program. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. Biomacromolecules 8, 2464-2475. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. 2015;32(5):170413. Macromolecules, 31, 7712-7716. 7 8 Carbohydrate Polymers, 68, 477-488. Journal Argentine Chemical Society 100 (2013) 25-34. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. With FITC-dextran 20 K, cumulative Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! HHS Vulnerability Disclosure, Help Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. The mechanism of the dextran-induced red blood cell Aggregation. hydrodynamic Front Microbiol. Experimental and numerical study on the mixed lubrication European Polymer Journal, 37, 619-622. Cell permeabilization using a non-thermal plasma - IOPscience Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Schematic illustration of transdermal delivery after the application of the microporation device. Polyelectrolytes as secondary calibration standards for aqueous SEC. (2012). Materiaux 1-5. Kasaai, Mohammad R. (2007). In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. Dextran Rheology of dextran solutions. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. CAS For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Ahn, G.Y., Eo, HS., Kim, D. et al. Ficoll and dextran vs. globular proteins as probes for testing ; Bos, G.W. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Garcia de la Torre, J.; Carrasco, B. ; De Jong, S.J. Webwhittier union high school district superintendent. 2009;8(2):1739. Expert Rev Med Devices. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. ; Tsuchiya, H.M.; Rist, C.E. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Springer Editions. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. ; van Nostrum, C.F. Arch Dermatol Res. Polymers | Free Full-Text | Confinement-Induced Fractionation and 1905/05) for their financial support. WebTry out PMC Labs and tell us what you think. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. National Library of Medicine A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Carbohydrate Research, 340, 811-826. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. (2007). Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Hydrodynamic Radius Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. dextran Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. He, L.; Niemeyer, B. By using this website, you agree to our In addition, we evaluated the regeneration of SC after microporation. Ibrahim O, Munavalli GS, Dover JS. Macromolecules. This study was supported by the LG Electronics. ; Aberle, T; Burchard, W. (2000). Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Pure Appl. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Each microporated sample was incubated in the skin culture medium. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. Thus, it is a common practice to express the frictional coefficient of rigid structures as. (2000). Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. If there are particles in the sample, the incident laser light gets scattered in all directions. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Huggins, Maurice L. (1942). (1999). Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. Viscotek GmbH, Europe. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. Critical parameters for gel permeation These studies were carried out at the Hisko (Gunpo, Korea). Molecular dynamics simulations on aqueous Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Drug Discov Today Technol. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. 2011;5(2):95132. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Kasaai, Mohammad R. (2012). The Mark-Houwink. Drug Deliv. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Viswanath, D.S. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Journal of Colloid Science, 31, 308-328. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. dextran The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. (2006). All branched polysaccharide types could be described approximately by the hyperbranching theory. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Nanoparticles in magnetic resonance imaging: from simple to dual Fluorescent dextran Learn More. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Biophysical Journal, 27, 177-181. (2004). Pharmaceut Sci Tech. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. For Research Use Only. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Carbohydrate Polymers, 74, 372-378. Hydrodynamic Exp Cell Res. 4h after microporation, the sizes of the micropore decreased slightly. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. The https:// ensures that you are connecting to the Stokes radius In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Epub 2012 Oct 18. Carbohydrate Polymers, 79, 380-390. 2006;3(3):37385. (1999). ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Pharm Res. Advances in Carbohydrate Analysis, 1, 63-144. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. FITC-dextran solution (600L) was added to the donor chamber. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Keywords: Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. 2. Food Sci Biotechnol. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. PubMedGoogle Scholar. Characterisation of insulin analogues therapeutically available to Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Polymer molecular weight distribution. Dextrans in Aqueous Solution. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. European Polymer Journal, 36, 2143-2146. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. ; Patel, T.R. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Polymers | Free Full-Text | Confinement-Induced Fractionation and J. Braz. WebR H Hydrodynamic radius [m] The basic DLS setup. For data requests, please contact the authors. The above data are not useful because it can not be compared with those obtained in this work. Dilute Solution. Analytical Chemistry. Crini, G. (2005). Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. 8600 Rockville Pike 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. (2003). Polymolecularity and polydispersity in Molecular weight determinations. 2013;3(5):3588605. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time.
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